Advancing Microfluidic Sample Preparation and Mass Spectrometry for Single Cell and Spatial Multiomics - Dr. Ying Zhu (08/18/2022, 12-1pm EST)

CASMS Webinar Announcement

Time:              Thursday, August 18, 2022 (12-1pm) US EST

Title:               Advancing Microfluidic Sample Preparation and Mass Spectrometry for Single Cell and Spatial Multiomics

Presenter:     Dr. Ying Zhu, Senior Research Scientist, Pacific Northwest National Laboratory

Moderator:    Prof. Tao Huan, The University of British Columbia

Panelists:      Prof. Yue Chen, University of Minnesota at Minneapolis

                       Dr. Naidong Weng, Janssen Pharmaceuticals R&D




Despite the rapid development and application of single-cell genomics and transcriptomics technologies have dramatically advanced our knowledge on the diversity and complexity of cellular populations, scientists increasingly recognize that gene sequence or RNA abundance only reflects part of the stories, because the cellular functions are directly implemented by proteins. Because of the lack global amplification method, large-scale detection and quantification of proteins in single cells have been a formidable challenge. In this webinar, I will give an introduction on several PNNL-developed technologies in sample preparation and mass spectrometry. I will show how these technologies can enable to precisely quantify >1000 proteins in single cells and to directly visualize the abundance of >3000 proteins on tissue sections at near single-cell resolution. I will highlight the advantage of miniaturized protein digestion based on nanoPOTS, as well as the use of ion mobility to improve the detection of low abundant proteins. Finally, I will describe how we extend single-cell proteomics into single-cell multiomics by efficiently splitting single-cell lysate into different nanoreactors based on a technology termed nanoSPLITS.


Short Biography of Dr. Ying Zhu:

Dr. Ying Zhu is a Senior Research Scientist at the Instrument Development Laboratory of Pacific Northwest National Laboratory. He has been leading a research team and focusing on the development of ultrasensitive and high throughput mass spectrometry technologies for single-cell and spatial omics analysis. Currently, he is the PI of a couple of NIH and DOE projects and is heavily involved in many NIH projects targeting to different diseases. 


Event Information

Event Date 08-18-2022 12:00 pm
Location Online